Stopped Flow Technique

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Yea Eun Lee 1H
Posts: 33
Joined: Fri Sep 29, 2017 7:06 am

Stopped Flow Technique

Postby Yea Eun Lee 1H » Wed Mar 07, 2018 4:21 pm

What is the role of the stopping syringe in the stopped flow technique? How is this technique supposed to measure the change of concentrations over time?

Gurvardaan Bal1L
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Re: Stopped Flow Technique

Postby Gurvardaan Bal1L » Wed Mar 07, 2018 11:00 pm

For stopped flow technique, you are basically mixing solutions of reactants together and observing their reaction over a course of a millisecond using spectroscopy to measure it. The stopping syringe, at least to my understanding, would prevent anymore reactant from being added into the reaction chamber. This technique is used for very small amounts of time, such as a millisecond or even attoseconds, and by using spectroscopy you can measure the change in concentration. With the change in concentration and the time interval, you can then calculate the rate.

David Zhou 1L
Posts: 61
Joined: Fri Sep 29, 2017 7:04 am

Re: Stopped Flow Technique

Postby David Zhou 1L » Fri Mar 09, 2018 4:44 pm

Attoseconds are much faster than what's controllable by a stopped syringe - they're 10^-18 seconds. That's a unit of time that's completely unheard of in science, except at quantum level.

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