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Nope it does not matter, although you have to be consistent. For example, if you start filling spin-ups in the 2px orbital, you have to continue filling spin-ups in the 2py and 2pz orbitals until you need to pair all the spins in the p-orbitals to complete the configuration.
According to Hund's Rule, electrons in the same subshell occupy different orbitals with parallel spin, but when electrons are initially added, it doesn't matter whether the spin is up or down, as long as they are all parallel.
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