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Hydrogen bonding sites are represented by and H paired to an N, O or F so it would look something like this O-H N-H F-H. These bonds would in turn bond to an O, N or F with a lone pair on the opposite antiparallel strand of DNA. The partial positive created on the H in the N,O or F-H bond will be attracted to the partial negative charge on the O,N and F with lone pairs, attracting them to each other and creating a Hydrogen bond. A GC pair has 3 Hydrogen bonds while AT pair only has 2 H bonds. This gives GC a higher boiling point because it contains more bonds, which requires more energy to break and in turn would require more heat energy to boil and break those bonds.
Potential hydrogen bonding sites of a molecule include the Hydrogens on the molecule as well as the lone pairs on hydrogen, oxygen, nitrogen, and fluorine molecules. For example, water has hydrogen bonding sites on the two hydrogen molecules and the two lone pairs on the oxygen, so it has four hydrogen bonding sites.
Do they F, O, and N atoms on a separate molecule have to be bonded directly to a H atom for Hydrogen bonding to be able to occur? For example, in benzoic acid C6H5COOH, there is an O doubled bonded to a C, so the O is not directly bonded to an H. Can this particular O still participate in Hydrogen bonding with an H from a different molecule, even though this O is not directly bonded to an H?
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