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In every case I can think of, enzymes act as catalysts by lowering the activation energy of the reaction and speeding up the reaction. Cells use a lot of energy, so it is in their best interest to evolve enzymes that will speed up their chemical processes as opposed to slowing them down. Enzymes and active sites can become denatured so that they will no longer bind and be able to act as a catalyst, but in general there are not enzymes that slow down reactions.
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